Abstract
Summary Protein content and patterns of protein, peroxidase and malate dehydrogenase isoenzymes in wounded tuber tissue from Solanum tuberosum L. (cv. SASKIA) with different anatomical wound-reactions are investigated. Periderm, callus and cutis forming tissues as well as tissues with reduced anatomical wound-reactions are compared. The protein content was enhanced in all wounded tissues. The rise in protein content of periderm-forming cells is twice that of the other tissues. In spite of different anatomical reactions the four tissues show alterations after disc-gel-electrophoresis: Two new protein bands appear additionally in the pattern of all tissues as compared with the intact tuber. In woundhealing material there is also a dedifferentiation of the persistent patterns and, moreover, a higher background staining on the gels. The pattern of malate dehydrogenase isoenzyme shows no change after wounding whereas peroxidase isoenzyme pattern is altered. In cutis forming cells two additional isoenzymes appear as compared with the pattern of intact tissue which shows 11 bands of peroxidase activity. The other three tissues are characterized by three additional isoenzymes. The present results are discussed with regard to different aspects of woundhealing.
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