Abstract
AbstractThis contribution describes the use of surface plasmon resonance (SPR) technique to fully characterize polymer‐based immunosensing surfaces. Bovine serum albumin (BSA), selected as model biomacromolecule probe, was covalently immobilized on a gold substrate via a poly(glycidyl methacrylate) (PGMA) thin film. Optical characterization was performed at the various steps of the sensor preparation and application. SPR measurements were performed first in static mode to characterize both PGMA and BSA films as well as in dynamic mode to monitor the immunocapture of antibovine serum albumin (a‐BSA) and further study the regenerability of the surface at the level of the antigen layer. Effect of the preparation method of PGMA films, based on either grafting‐onto or grafting‐from approaches, on the immunosensor efficiency was studied. In all cases, the sensing surface could be efficiently regenerated by using an acidic glycine solution without noticeable loss of activity demonstrating the usefulness of poly(glycidyl methacrylate) spacers. Copyright © 2010 John Wiley & Sons, Ltd.
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