Proteinases of kilka Clupeonella cultriventris of the Rybinsk Reservoir

Abstract

The effect of temperature on activity of proteinases of the stomach mucosa, intestinal mucosa, chime, and the entire body of kilka Cluperonella cultriventris is described. Significant differences are revealed in temperature characteristics (to-function, Q10, and Eact) of the investigated enzymes. The temperature has a larger influence on the activity of intestinal proteinases than the activity of proteinases of the stomach, chime, and the whole kilka organism. The lowest value of Eact within the temperature range of life of this species is characteristic of chime proteinases. Hemoglobinlytic proteinases of various tissues of kilka that substantiate, at pH 3.0 and 5.0 (the action zone of cathespsins), the processes of induced autolysis contribute to efficient alimentation of other fish species. The assumption is made that a high activity level of proteinases of the digestive tract, comparable with that of other fish species of the upper Volga, enables kilka that have assimilated to the Rybinsk Reservoir to feed efficiently within the temperature range characteristic of the water-bodies of this zone.