Abstract
Synthesis of specific proteins in adenovirus-infected HeLa cells was studied utilizing the amino acid analog p-fluorophenylalanine. Virus multiplication and the production of specific soluble antigens could be completely suppressed by addition of this compound. Two distinct stages were revealed within the virus eclipse period when the presence of analog prevented virus synthesis. The first or early effect of p-fluorophenylalanine appeared to result from interference with production of functional enzymes necessary for synthesis of virus structural protein. An overall picture of the temporal relationships between DNA, protein, and virus synthesis was obtained by conducting inhibition studies in parallel employing 5-fluorodeoxyuridine, an inhibitor of thymidilate synthetase, as well as p-fluorophenylalanine. These studies revealed that virus DNA synthesis preceded the production of virus-specific protein by approximately 2 hours and the synthesis of mature infectious virus by about 4 hours.
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