Abstract
Protein synthesis in a rat brain mitochondrial preparation purified by centrifugation through a ficoll gradient is inhibited by chloramphenicol and oxytetracycline but not by cycloheximide. This establishes that rat brain mitochondrial protein synthesis is similar to liver mitochondria in its sensitivity to these antibiotics. The possible nature of the cyclo-heximide-sensitive protein synthesis found with other brain mitochondrial preparations is discussed.
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