Protein Synthesis in Dormant and Non‐Dormant Cocklebur Seed Segments

Abstract

AbstractUsing the axial and cotyledonary segments of lower cocklebur (Xanthium pensylvanicum Wallr.) seeds, protein synthesis as shown by incorporation of radioactive leucine was examined in relation to their dormant status. During the first 9 h of water imbibition, the protein synthesis was higher in the dormant axes than in the non‐dormant, after‐ ripened ones. When imbibed for more than 12 h non‐dormant axes had a higher activity than dormant ones. This was also the case with the cotyledonary segments.Cyctoheximide, an inhibitor of protein synthesis, blocked protein synthesis in the axial tissue regardless of its dormant status, and thereby inhibited germination of the non‐dormant seeds. In the dormant seeds, however, cycloheximide at 3 mM slightly stimulated germination without stimulating the C2H4 production. Based on these results, it is suggested that in cocklebur seeds there may be some proteinaceous system which is involved in the maintenance of dormancy.