Abstract
1. One of the unicellular Chlorophyceae, Chlorella sp., was grown in pure culture in a nutrient solution to which combined nitrogen was supplied in inorganic combination, either as calcium nitrate or ammonium sulphate. 2. Cultures were grown in diffuse light and in the total absence of light for 105 or 235 days. 3. Quantitative determinations were made of the volume, dry weight, and total nitrogen content of the growth obtained. 4. The data obtained offer strong evidence indicating that Chlorella sp. can synthesize proteins in total darkness when nitrogen is supplied in inorganic combination. 5. No evidence of free nitrogen fixation by Chlorella sp. was obtained.
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