Abstract
The characteristics of an in vitro amino acid-incorporating system prepared from germinated Peronospora tabacina conidia are described. Material sedimenting at 20,000 g and at 117,000 g was found to possess significant amino acid-incorporating activity. Although germination is accompanied by an increase in the activity of both these fractions, if RNA synthesis is inhibited during germination, activity increases only in the 20,000 g fraction. Since germ tube formation occurs in the absence of RNA synthesis, it is suggested that the protein required for germ tube formation is largely synthesized by this 20,000 g fraction. The increase in amino acid-incorporating activity in this fraction begins during the preparation of conidia at 0o) and prior to the start of incubation at 15 o and may result from the removal of some factor during washing.
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