Protein synthesis by ribosomes from rat kidney cortex: effect of aldosterone and bilateral adrenalectomy.

Abstract

Experiments with renal cortical ribosomes from adrenalectomized rats have shown that the incorporation of phenylalanine into polypeptide by ribosome preparations from aldosterone-treated animals is greater than incorporation by control ribosomes by a range of 20–24%. When incorporation was directed by poly U the hormone-stimulated ribosomes produced a range of 15–32% greater incorporation than control ribosomes. This latter difference might be due to a change in the functional capacity of the ribosomes induced by the hormone treatment since the poly U directed binding of phenylalanyl-tRNA to the ribosomes from aldosterone-treated rats was greater than that to the control ones by a range of 16–35%. Furthermore, ribosomes from the aldosterone-stimulated animals had an average of 26% more ribonuclease activity than the control ribosomes.