Protein synthesis by ribosomes from eggs of normal and thiotepa-chemosterilized house flies.

Abstract

The ribosomes from the eggs of normal and thiotepasterilized Musca domestica L. were characterized after sucrose density gradient (SDG) separation by their ability to induce protein synthesis in vitro. Although profiles after SDG separation were similar near the time of oviposition, the normal formation of heavier aggregates did not occur in chemosterilized eggs upon incubation. In a cell-free system, the ribosomes from normal eggs were 1.6 times more active in inducing protein synthesis than the ribosomes from chemosterilized eggs.