Abstract
The proteins synthesized in molluscum contagiosum virus (MCV) infected cells during one hour pulse-labelling experiments with14C amino acids were separated by sodium dodecyl sulphate acrylamide gel electrophoresis and analysed by autoradiography. In addition, the surface proteins of infected cells were labelled with125I and then analysed with the same procedure in order to detect differences in the spatial arrangement of proteins during the cytopathic effect (c.p.e.) and compared with the controls. In both types of experiments the electrophoretic pattern of human embryonic cells did not show qualitative differences between infected cells and control cells. This indicated that MCV fails to express, among the “early” events, the function(s) necessary to switch off host protein synthesis. The iodination of the cell surface showed that the cytopathic cell alteration did not correspond with a variation in the protein distribution on the cell surface.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.