Abstract
Our group has previously established a strategy utilizing fluorescence lifetime probesto image membrane protein supercomplex (SC) formation in situ. We showed that a probe at the interface between individualmitochondrial respiratory complexes exhibits a decreased fluorescence lifetime when a supercomplex is formed. This is caused by electrostatic interactions with the adjacent proteins. Fluorescence lifetime imaging microscopy (FLIM) records the resulting decrease of the lifetime of the SC-probe. Here we present the details of our method for performingSC-FLIM, including theevaluation of fluorescence lifetimes from the FLIM images. Tovalidatethe feasibility of the technique for monitoring adaptive SC formation, we compare data obtained under different metabolic conditions. The results confirm that SC formation is dynamic.
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