Abstract
Ionic liquids have been extensively used as environmentally friendly solvents for enzymatic reactions and other biological systems. Understanding the mechanism of how ionic liquids affect protein stability is crucial for the biological reaction processes and protein storage using ionic liquids as solvents. Although effects of ionic liquids on protein stability have been studied, equilibrium thermodynamics of protein stability in ionic liquids has not been quantitatively measured. Herein, we utilized 19F NMR to measure the equilibrium thermodynamics of protein stability in ionic liquid [C4-mim]Br. Our results show that proteins are significantly destabilized in [C4-mim]Br ionic liquids. Our results suggest that 19F NMR provides a simple and effective way to study the thermodynamics of protein stability in ionic liquids. 19F NMR will be applicable to facilitate the protein-protein interaction study in ionic liquids. Graphical abstract.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.