Abstract
AbstractDynamic field gradient focusing uses an electric field gradient to separate and concentrate proteins in native buffers. A prototype preparative‐scale dynamic field gradient focusing apparatus reproducibly separated hemoglobin and bovine serum albumin with a mean resolution of 2.64 ± 0.503. Run‐to‐run variations in the hemoglobin's focal point and peak width appeared to be related to fluctuations in the shape of the electric field, rather than the 5% accuracy of the pump that provided the counter‐flow in the separation annulus. The variation in the electric field gradient was probably due to the formation and expansion of an ion‐depleted region at the top of the separation annulus.
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