Abstract
A quantitative investigation of the activity in “nuclear bodies” in spleen cells from immunized animals after injection of tritiated leucine was performed by means of high resolution autoradiography, using the density distribution method of Salpeter et al. [21]. The activity in nuclear bodies was found to be significantly higher than in the surrounding nucleoplasm. As a previous study demonstrated that the mean nuclear activity varied with time after injection, the kinetics of labelling of nuclear bodies was compared with that of nuclei. The curve fitted the distribution of the renewal of a pool. These data bring evidence that nuclear bodies contain proteins and are sites of protein renewal. This is consistent with previous ultrastructural cytochemical studies. This work is taken as an example of the usefulness of this method to investigate the DNA, RNA, and protein content of other organelles and inclusions in the cell nucleus after exposure to appropriate precursors.
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