Protein Purification Protocols

Abstract

General Strategies Shawn Doonan and Paul Cutler Preparation of Extracts From Animal Tissues J. Mark Skehel Protein Extraction From Plant Tissues Roger J. Fido, E. N. Clare Mills, Neil M. Rigby, and Peter R. Shewry Extraction of Recombinant Protein From Bacteria Anne F. McGettrick and D. Margaret Worrall Protein Extraction From Fungi Paul D. Bridge, Tetsuo Kokubun, and Monique S. J. Simmonds Subcellular Fractionation of Animal Tissues Norma M. Ryan Subcellular Fractionation of Plant Tissues: Isolation of Plastids and Mitochondria Alyson K. Tobin and Caroline G. Bowsher The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds William S. Pierpoint Avoidance of Proteolysis in Extracts Robert J. Beynon and Simon Oliver (Revised by Paul Cutler) Concentration of Extracts Shawn Doonan Making and Changing Buffers Shawn Doonan Purification and Concentration by Ultrafiltration Paul Schratter (Revised by Paul Cutler) Bulk Purification by Fractional Precipitation Shawn Doonan Ion-Exchange Chromatography Chris Selkirk Hydrophobic Interaction Chromatography Paul A. O'Farrell Affinity Chromatography Paul Cutler Dye-Ligand Affinity Chromatography Anne F. McGettrick and D. Margaret Worrall Lectin Affinity Chromatography Iris West and Owen Goldring (Revised by Paul Cutler) Immunoaffinity Chromatography Paul Cutler Immobilized Metal-Ion Affinity Chromatography Tai-Tung Yip and T. William Hutchens (Revised by Paul Cutler) Chromatography on Hydroxyapatite Shawn Doonan Thiophilic Affinity Chromatography and Related Methods Paul Matejtschuk Affinity Precipitation Methods Jane A. Irwin and Keith F. Tipton Isoelectric Focusing Reiner Westermeier Chromatofocusing Timothy J. Mantle and Patricia Noone Size-Exclusion Chromatography Paul Cutler Fast Protein Liquid Chromatography David Sheehan and Siobhan O'Sullivan Reversed-Phase Chromatography of Proteins William A. Neville Extraction of Membrane Proteins Kay Ohlendieck Removal of Detergent From Protein Fractions Kay Ohlendieck Purification of Membrane Proteins Kay Ohlendieck Lyophilization of Proteins Ciaran O'Fagain Storage of Pure Proteins Ciaran O'Fagain Electroelution of Proteins From Polyacrylamide Gels Michael J. Dunn Electroblotting of Proteins From Polyacrylamide Gels Michael J. Dunn Two-Dimensional Polyacrylamide Gel Electrophoresis for Proteome Analyses Neil A. Jones Microscale Solution Isoelectrofocusing: A Sample Prefractionation Method for Comprehensive Proteome Analysis Xun Zuo and David W. Speicher Practical Column Chromatography Shawn Doonan Detection Methods Jacek Mozdzanowski and Sudhir Burman Peptide Proteomics Dean E. McNulty and J. Randall Slemmon Multidimensional Liquid Chromatography of Proteins Rod Watson and Tim Nadler Mass Spectrometry David J. Bell Purification of Therapeutic Proteins Julian Bonnerjea Purification Process Scale-Up Karl Prince and Martin Smith Index