Abstract
Counteracting Chromatographic Electrophoresis (CACE) is a novel purification technique that focuses the target protein into a thin band at the interface separating two gel media which differ sharply in internal porosity. This article validates a criterion that predicts protein focusing by CACE, using the colored proteins-ferritin, hemoglobin and myoglobin. Fair comparison is shown between the theory and the experiment. Data from the past literature which were reported to exhibit nonconformity have also been shown to agree fairly well with the present model. Variations in protein focusing conditions can be partly explained by protein-ion binding.
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