Abstract
Long-term exposure to commonly used pesticides (endosulfan, malathion, chlorpjoifos) induce synthesis of stress proteins in the body tissues to combat anxiety. From the protein extract of liver tissues of the fish, Labeo rohita, nine protein fractions within range of 22.4 to 138 kDa were visible in the electropherogram obtained after SDS-PAGE, The fish appeared sensitive to the exposure with increase in sublethal concentrations of the pesticides. A new protein fraction of 77.6 kDa appeared on exposure to all the treatments of the three pesticides except 0.0001-ppm endosuifaiL The 138,112.2, 70.8 kDa fractions were deleted from the protein profile in all the three 0.0001, 0.0005 and O.OOlppm treatments of chlorpyrifos and malathion. The pattern of expression may be unique to a specific pesticide, 125.9 k Da fraction resolved only in endosulfan treatment. The new protein fractions (64.6 and 30.2 kDa) persisted even after the withdrawal of toxicants.
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