Protein profiling of regenerative and non regenerative callus cultures of Glossonema varians: a rare, endemic and edible plant of Indian Thar Desert

Abstract

Glossonema varians is a rare and endemic plant species of arid and semiarid region of Rajasthan, used as edible plant in Thar Desert. The plant is facing serious threat of extinction from its natural habitat due to overexploitation and slow propagation in natural conditions. Moreover, plant is known for its nutritional values, hence present manuscript is focused on protein profiling of G. varians. The internodal shoot explants were taken from in vitro grown cultures and inoculated on Murashige and Skoog’s (MS) medium augmented with 25 mg each of citric acid, arginine, adenine and 50 mg of ascorbic acid with different concentrations of PGRs for initiation of callus culture. A combination of 2, 4-D (2, 4-dichlorophenoxy acetic acid) and NAA (α-naphthalene acetic acid) was found to be suitable for optimum growth of callus. Regeneration of shoot buds was observed on MS medium augmented with a combination of BAP (6-benzylaminopurine), Kn (Kinetin), NAA and iP (N6-[2-isopentenyl] adenine). Comparative biochemical characterization of regenerative and non- regenerative callus has been done through in vitro quantification and protein profile has also been done by SDS-PAGE.