Protein profiling in unlocking the basis of varicocele-associated infertility.

Manesh Kumar Panner Selvam,Saradha Baskaran,Ashok Agarwal,Ralf Henkel

doi:10.1111/and.13645

Manesh Kumar Panner Selvam, Saradha Baskaran + Show 2 more

Open Access

https://doi.org/10.1111/and.13645

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Journal: Andrologia	Publication Date: May 26, 2020
Citations: 9	License type: cc-by

Affiliation: Cleveland Clinic, University of the Western Cape

Abstract

Varicocele is one of the major causes of male infertility and has a negative impact on spermatogenesis. The conventional semen analysis does not reveal the underlying subcellular mechanisms associated with defects in spermatozoa. Proteomics and bioinformatics analysis can be used to identify the molecular aetiologies associated with poor semen quality in varicocele patients. Mitochondrial dysfunction has been identified as the main factor affecting normal physiological functions of spermatozoa. This article discusses the proteomic studies of spermatozoa and seminal plasma in varicocele patients. Proteomics can identify potential spermatozoa and seminal plasma biomarkers in varicocele-mediated male infertility. In future, these protein biomarkers can be useful in the development of noninvasive diagnostic and therapeutic strategies for varicocele patients.

Highlights

The South African rocky intertidal and shallow subtidal zones have both an abundance and diversity of nongeniculate coralline red algae with three (Corallinales, Hapalidiales, Sporolithales; Stephenson and Stephenson 1972; Maneveldt et al 2008) of the four orders (Corallinales, Corallinapetrales, Hapalidiales, Sporolithales; Jeong et al 2020) represented
The RAxML and Bayesian analyses, for both the psbA (Figure 2) and rbcL (Figure 3) genes were congruent in resolving Chamberlainium capense sp. nov., Chamberlainium glebosum sp. nov. and Chamberlainium occidentale sp. nov. in a monophyletic clade in Chamberlainoideae with full support (100% BS/ 1 PP)
In the psbA tree (Figure 2), the clade formed by C. cochleare, C. capense, C. glebosum and C. occidentale is weakly supported (83/0.79) as sister to two northeast Pacific species, C. tumidum and C. decipiens; in the rbcL tree (Figure 3) the relationship of these northeast Pacific species is unresolved with respect to the other Chamberlainium species

Summary

Introduction

The South African rocky intertidal and shallow subtidal zones have both an abundance and diversity of nongeniculate coralline red algae with three (Corallinales, Hapalidiales, Sporolithales; Stephenson and Stephenson 1972; Maneveldt et al 2008) of the four orders (Corallinales, Corallinapetrales, Hapalidiales, Sporolithales; Jeong et al 2020) represented. South Africa has representative species from more than half of the currently recognized extant genera of non-geniculate coralline red algae, with the order Corallinales best represented (Maneveldt et al 2016). Included in this order are species previously placed in Spongites and the recently erected Chamberlainium that are widespread and ecologically important in South Africa (Chamberlain 1993; Keats et al 1993; Maneveldt and Keats 2008; Puckree-Padua et al 2020b). The former of these characters no longer holds true for separating the genera morpho-anatomically (Puckree-Padua et al 2020b)

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