Abstract
The aim of this study was to evaluate the association between proteins in the seminal plasma of tambaqui Colossoma macropomum (Cuvier, 1818) with seminal quality indicators after thawing. The semen was cryopreserved with a dilution based on BTS with 8% DMSO. A 200 µL sample of semen from each animal was diluted in 800 µL BTS, centrifuged at 800 rpm, and the supernatant was cryopreserved to further analyze of the protein profile of seminal plasma through one-dimensional electrophoresis (SDS-PAGE). After 15 days of cryopreservation, a cryopreserved semen straw was thawed to analyze both qualitative and quantitative parameters. When considering all collections, the SDS-PAGE identified 15 protein bands in the seminal plasma of tambaqui. When the interaction (presence or absence) between proteins observed in the seminal plasma and the post thawed spermatic parameters was evaluated, we observed a great influence of the presence of proteins on spermatic quality. A greater (P<0.05) fertilization rate was observed with the presence of proteins 12, 34, 44, 85, and 90 kDa. Proteins in seminal plasma of tambaqui influenced the spermatic quality after thawing, and thus, they can be utilized as an indicator of sperm quality, especially the proteins with a molecular weight ≤ 50 kDa.
Highlights
Gamete conservation is an important tool for fish reproduction and of great interest for fish-farming (Magnotti et al, 2018)
When the interaction of proteins found in the seminal plasma and the spermatic parameters of C. macropomum frozen with DMSO was evaluated, an effect of proteins on spermatic quality was observed (Tables 2 and 3)
Several proteins of seminal plasma have been identified and characterized (Moreau and Manjunath, 1999), and others have been associated with fertility in different species (Ayyagari et al, 1987; Kraus et al, 2001)
Summary
Gamete conservation is an important tool for fish reproduction and of great interest for fish-farming (Magnotti et al, 2018). The protein composition of the seminal plasma should be deemed as an important factor that influences fertility in males (Autiero et al, 1991; Strzeżek et al, 2005) a glycoprotein in the seminal plasma with a high molecular weight (120 kDa) was identified as a factor in the immobilization of spermatozoids (Mochida et al, 1999, 2002) in Nile tilapia (Oreochromis niloticus) Some of these proteins can be used as biochemical markers to identify individuals with higher or lower potential for fertility and freezing of semen (Jobim et al, 2004; Zilli et al, 2005; Asadpour et al, 2007)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
