Abstract
<p>Indonesia had a high number of malaria cases where the treatments are only perceived by 10% of patients in health facilities. The resistance of <em>Plasmodium</em> sp. to drugs causes the difficulty of controlling malaria. The use of irradiation to produce malaria vaccine material is being explored until nowadays. In previously study that dose rate of gamma irradiation delivered to parasite was at the low dose rate (380 Gy/hour) which produced several doses that not be able to attenuate the parasite. This research aimed to increase dose rate (740 Gy/hour) and to determine the dose irradiation effect of 150-250 Gy to the protein profile, erythrocyte and leukocyte numbers in mouse blood infected by <em>P. berghei</em> erythrocytic stage. Results showed that there was an influence of dose rate of 740 Gy/hour for doses of 150-250 Gy in treated mice and no difference in erythrocyte, leucocyte and body weight with negative control. Dose of 150 Gy was the optimal dose to attenuate parasites, whereas doses more than 150 Gy effectively killed the parasites. The changes of protein profile marked by the appearance of higher number of bands at molecular weight of 30-37 kDa that predicted as merozoite surface protein of <em>P. berghei</em> and/or antibodies elicited in mouse.</p><p><strong>How to Cite</strong></p><p>Rosadi, I., Syaifudin, M., &amp; Elfidasari, D. (2016). Protein Profile and Hematological Parameters of Mice post Injected with Irradiated Plasmodium berghei. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(2), 155-164</p>
Highlights
Southeast Asia is one of the regions with high mortality rate of malaria
We developed our research about Plasmodium berghei that has a level of violence similar to P. falciparum which causes death in the blood stage
Plasmodium berghei Breeding P. berghei harvested after reaching more than 30% parasitaemia, with an average of 34.03% on day 14 (Table 1)
Summary
Southeast Asia is one of the regions with high mortality rate of malaria. There are 28 million cases of malaria found in Southeast Asia from 216 million cases in the world in 2010 (Autino et al, 2012). Indonesia as the island nation that located in the South-East Asian region has quite high prevalence (number of events) of malaria. It is noted that Indonesia have a number of malaria paining of 1.85 per 1,000 inhabitants in 2009. Malaria is a disease that can be found in animals and human which caused by protozoa of obligate intracellular of Plasmodium infection. That infecting human namely a Plasmodium ovale, Plasmodium malariae, Plasmodium vivax and Plasmodium falciparum. Plasmodium falciparum is the most dangerous parasite because it can cause of death in children and pregnant women (Zuzarte-Luis, Mota, & Vigário 2014; Lamikanra et al, 2007; Krishna et al, 2015)
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