Abstract
A study was made of whole soybeans, isolated protein bodies and a fraction consisting of globoid inclusions separated from protein bodies by centrifugation in order to observe the cellular location of phytate and to determine any indication of protein-phytate interaction within the soybean cell. Microstructural and chemical evidence demonstrated phytic acid to be located in the protein bodies of the soybean, likely in the form of a soluble protein-phytate salt with significant amounts specifically deposited in globoid inclusions. The probable existence of a protein-cation-phytate complex in situ was shown by gel filtration in which calcium and phosphorus was found to coelute with soluble proteins. The proteins involved in this interaction were tentatively identified as the 7S soybean fraction.
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