Abstract
Crude microsomal membranes prepared from Chlorella vulgaris catalyze the synthesis of the dolichol-bound monosaccharides Dol-P-Man, Dol-P-Glc, and Dol-PP-GlcNAc. The mannosyl transferase catalyzing the transfer of mannose from GDP-Man to Dol-P increases continuously in the light-phase of the cell cycle in parallel to total protein. The rate of in vivo incorporation of [ 14C]glucosamine into glycoproteins increases during the total Chlorella cell cycle; during early S-phase the relative increase based on total protein is lowest. Tunicamycin inhibits glucosamine incorporation into trichloroacetic acid (TCA) insoluble material by 60%, whereas protein-synthesis is not affected. The release of autospores is almost completely inhibited by tunicamycin (20 μg/ml), when the antibiotic is added to a synchronous culture before the S-phase. Glycoproteins seem to be required for the initiation of DNA-synthesis in Chlorella, as has been postulated previously for other cells.
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