Abstract
Abstract— Protein metabolism of goldfish brain was studied in vivo by means of intraperitoneal or intracranial injections of [3H]leucine and compared with concomitant studies in the mouse. Heterogeneity of turnover values was observed. Long turnover times were seen relative to other organs examined. The free amino acid pools of goldfish brain were determined, and the fate of tritium from labelled leucine was followed at various times after injection.Following ‘chasing’ with large amounts of unlabelled leucine or protein inhibitors shortly after isotope injection, further incorporation was arrested, but examination of the labelled protein over a period of 2 weeks indicated a slow decay, similar to that seen without ‘chasing’.Possible use of ‘pulse‐chase’ experiments in vivo in animals is discussed in relation to behavioural studies.
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