Abstract
Chitosan binds to negatively charged tripolyphosphate (TPP) by an electrostatic interaction driven by its positively charged amino group. This interaction allows developing stable nanoparticles suitable as a carrier and controlled release system for drugs and vaccines. We study the effect of chitosan nanoparticles (CNp) on the uptake and antigen presentation of the model protein hen-egg white lysozyme (HEL) to peritoneal macrophages isolated from mice. Results showed that after four hours of pre-incubation with a T-cell hybridoma line cocultured with murine peritoneal macrophages, only trace amounts of IL-2 were detected in treatments with HEL alone, whereas cocultures treated with HEL-CNp had already reached maximum IL-2 expression. Confocal microscopy studies showed that CNp had a higher uptake rate of the fluorescently labelled protein than the protein itself after 30 min of incubation with the peritoneal macrophages. Our results suggest that CNp system is a potential candidate for an oral vaccine delivery system.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
