Abstract
Virus-host interaction is a tug of war between pathogenesis and immunity, followed by either activating the host immune defense system to eliminate virus or manipulating host immune control mechanisms to survive and facilitate virus propagation. Comprehensive knowledge of interactions between host and viral proteins might provide hints for developing novel antiviral strategies. To gain a more detailed knowledge of the interactions with porcine circovirus type 2 capsid protein, we employed a coimmunoprecipitation combined with liquid chromatography mass spectrometry (LC-MS) approach and 222 putative PCV2 Cap-interacting host proteins were identified in the infected porcine kidney (PK-15) cells. Further, a protein-protein interactions (PPIs) network was plotted, and the PCV2 Cap-interacting host proteins were potentially involved in protein binding, DNA transcription, metabolism and innate immune response based on the gene ontology annotation and Kyoto Encyclopedia of Genes and Genomes database enrichment. Verification in vitro assay demonstrated that eight cellular proteins, namely heterogeneous nuclear ribonucleoprotein C, nucleophosmin-1, DEAD-box RNA helicase 21, importin β3, eukaryotic translation initiation factor 4A2, snail family transcriptional repressor 2, MX dynamin like GTPase 2, and intermediate chain 1 interacted with PCV2 Cap. Thus, this work effectively provides useful protein-related information to facilitate further investigation of the underlying mechanism of PCV2 infection and pathogenesis.
Highlights
Porcine circovirus (PCV) belongs to the genus Circovirus of the family Circoviridae (King et al, 2011)
To identify the potential host proteins binding to Porcine circovirus 2 (PCV2) capsid protein (Cap) in the infected PK-15 cells, we performed coimmunoprecipitation assays combined with liquid chromatography mass spectrometry (LC-MS) in PK-15 cells with or without PCV2 infection
47 proteins were present in mock cell lysate and were not considered for further interpretation and remaining, 222 differentially expressed cellular proteins were considered as novel cellular candidates interacting with PCV2 Cap in the infected PK-15 cells (Supplementary Table S2)
Summary
Porcine circovirus (PCV) belongs to the genus Circovirus of the family Circoviridae (King et al, 2011). It is a small, icosahedral, non-enveloped virus and its genome is a single-stranded, closedcircular DNA (ssDNA) of 1.7 to 2.0 kb in length (Tischer et al, 1982; King et al, 2011). PCV3 was identified and shown to be associated with porcine dermatitis, reproductive failure, and nephropathy syndrome (Phan et al, 2016; Palinski et al, 2017; Jiang et al, 2019). PCV undoubtedly causes enormous loss to pig industry worldwide as well as to the global economy
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