Protein Insufficiency Aggravates the Enhanced Lipid Peroxidation and Reduced Activities of Antioxidative Enzymes in Rats Fed Diets High in Polyunsaturated Fat

Abstract

To investigate the effects of dietary protein and polyunsaturated fat levels on tissue lipid peroxidation and antioxidative enzymes, Long-Evans male weanling rats were fed either an 8% lactalbumin diet containing 2% (L2), 5% (L5), 10% (L10), 15% (L15) or 20% (L20) soybean oil or a 20% lactalbumin diet containing 5% (N5) or 20% (N20) soybean oil for 8 weeks. The tissue thiobarbituric acid-reactive substances (TBARS) concentrations of the L2 group were similar to those of the N5 group except in plasma in which they were higher. The L5 group generally showed tissue TBARS concentrations comparable to the N20 group. Gradually increasing the dietary soybean oil level in the low protein diet further increased the tissue TBARS concentrations. The L20 group had significantly higher TBARS in RBC, liver, heart, kidney and muscle than the N20 group. The low protein-fed groups had lower activities of glutathione peroxidase (EC 1.11.1.9), superoxide dismutase (EC 1.15.1.1) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) in liver and catalase (EC 1.11.1.6) in RBC than the N5 group. Compared with the N5 group, the N20 group also showed higher TBARS concentrations and lower activities of certain antioxidative enzymes in some tissues. The antioxidative enzyme activities decreased more drastically with the increasing dietary soybean oil level in the low protein-fed groups than in those fed a normal level of protein. Supplementation of 150 mg/kg of all-rac-α-tocopheryl acetate to the L15 diet slightly decreased the TBARS in plasma, heart and liver and restored the depressed activities of RBC superoxide dismutase and catalase. The results indicated that insufficiency of dietary protein aggravates the enhanced production of TBARS and the reduced activities of antioxidant enzyme in rats fed a high soybean oil diet.