Abstract
Free-standing films are constituted by two monolayers of surfactant molecules surrounding an internal thin water layer, reduced to the ultimate hydration water molecules in the so-called newton black films (NBF). We report in this paper, the insertion of a soluble protein within an NBF made from soluble surfactants, leading to the formation of a dense 2D monolayer of proteins. In a second step, we show that this insertion method could also be used for phospholipidic films, for which the internal structures are close to that of biological membranes
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