Abstract
This is the first report about flaxseed protein hydrolysates applied as media supplements in CHO cell culture. The hydrolysates were produced by three separate enzymatic digestions of proteins isolated from flaxseed oil cake. The enzymes used were Alcalase, Neutrase, and Protamex, and the most efficient hydrolysis was achieved with Alcalase. The three hydrolysates were first tested as a partial substitute for serum in basal media in order to evaluate their effects on the adherent IgG-producing CHO cell line. The cells that grew in such media reached higher density than the cells in media supplemented with serum only. Consequently, the increased cell number improved the final IgG titer. In the next experiment, the impact of hydrolysates was evaluated in suspension CHO culture adapted to chemically defined media. In this preliminary investigation, the cells showed no response to the hydrolysate addition concerning the growth rate and productivity. Despite this outcome, we speculate that low molecular mass components in the hydrolysates, besides nutritive, may have a cell-protective function.
Highlights
Animal cells are a complex expression system applied in a wide range of life science research as well as in industrial production of various biologicals
We describe adherent CHO DP-12 cell growth and monoclonal antibody production in Dulbecco’s Modified Eagle’s Medium (DMEM), where serum is partially replaced by flaxseed protein hydrolysates
Since the hydrolysates were made from a crude material that currently has no specific purpose in animal cell technology, it seemed reasonable to screen for their potential benefit in that particular area [6]
Summary
Animal cells are a complex expression system applied in a wide range of life science research as well as in industrial production of various biologicals. Due to its biological source, serum has undefined composition, which can lead to extended downstream processing and result in batch-to-batch variability, and even contamination with blood-borne pathogens. This made many regulatory authorities recommend avoiding ingredients of animal origin in bioprocessing production as much as possible. An adjustment can be made by application of plant-derived protein hydrolysates. These kinds of hydrolysates have been used occasionally for almost five decades for the reduction or replacement of FBS from basal media, such as DMEM [2,3]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
