Abstract
Acinetobacter baumannii (Acb) is an opportunistic and nosocomial pathogen that ussualy found in clinical specimen from patients with intensive care. The pathogenic mechanism of this bacteria are not fully elucidated especially potential activity of its protein as hemaglutinin and adhesion molecul. The aim of this study is to evaluate the  role of 16 kDa fimbriae protein from urnary tract infection (UTI) patient as hemaglutinin and adhesion molecule. Usingexplorative design this study was started by isolation Acb bacteria from urine of patient that had been determine as UTI clinically and laboratory. After identification this bacteria by microbact system hemaglutination test and isolation  of its fimbriae fraction, 12.5% SDS-PAGE had been used to isolated fimbriae protein, following assay in vitro to adhesion test..The study showed that the 16 kDa fimbriae protein of Acb bacteria was a hemaglutinin protein that could agglutinate 0.5% mice erythrocytes (1/32), and human blood group O erythrocytes (1/8). Hemaglutination test were negative onerythrocytes from rat, guinea pig, sheep, and human blood group A, B. The 16 kDa fimbriae protein (AF16) was also adhesion protein that had been revealed by its activity to adherenceto receptor of mice enterocytes. The increasing dose of AF16 molecules will decrease the amount of Acb bacteria to adherence to enterocytes (p<0.05). The fimbriae of Acb is maybe classified P type. Key words:Acinetobacter baumannii, fimbriae, hemaglutinin protein, adhesion protein
Highlights
Bakteri Acinetobacter baumannii (Acb) termasuk golongan bakteri ‘nonfermenter’ berbentuk batang atau kokobasil Gram negatif, secara alamiah dapat dijumpai di lingkungan, tanah, air dan kotoran [1], bahkan terdapat di mukosa farings dan kulit yang sehat [2]
The aim of this study is to evaluate the role of 16 kDa fimbriae protein from urnary tract infection (UTI) patient as hemaglutinin and adhesion molecule
After identification this bacteria by microbact system hemaglutination test and isolation of its fimbriae fraction, 12.5% SDS-PAGE had been used to isolated fimbriae protein, following assay in vitro to adhesion test
Summary
Spesimen secara aseptik ditanam pada medium agar MacConkey dan diinkubasi pada suhu 37°C, 18-24 jam. Isolat sel bakteri utuh Acb dilakukan uji hemaglutinasi (lihat prosedur), dipilih isolat dengan hasil uji hemaglutinasi positif dan dibuat kultur stok dengan cara disimpan pada suhu -70°C atau -20°C dalam medium cair Brain Heart Infusion (BHI) yang mengandung gliserin 22%. Kemudian ditanam pada medium agar MacConkey dan di inkubasi pada almari inkubator suhu 37°C selama 24 jam. Koloni yang tumbuh cek kembali dengan pewarnaan Gram, uji katalase, oksidase. Kemudian dilakukan perbanyakan dan dibiakkan pada medium yang dapat memperkaya pertumbuhan fimbria yaitu medium agar bifasik TCG yang terdiri dari medium padat agar miring TCG (thioproline carbonate glutamate) dan medium cair BHI kemudian diinkubasi pada suhu 37°C selama 2 X 24 jam, koloni yang tumbuh dipanen dan disiapkan untuk uji. Uji HA dilakukan menurut McGarey dan Allred dengan sedikit modifikasi [24]
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