Abstract
Protein is a substantial nutrition that essentially required by human. Spirulina platensis (Spp), well known as protein source could be a significant source to be used for many industrial applications. This study was investigated the effectiveness of ultrasound assisted extraction (UAE) method for protein extraction from Spp at various composition of solvent mixture and extraction time. Ethanol and mixture of methanol-ethanol were used as solvent. Extraction was conducted by varying ratios of solvent to biomass at 10:1, 12.5:1, and 15:1 (v/w), and extraction time (20, 35, and 50 min). Optimum protein recovery from dry Spp was 42.55 ± 0.43% obtained by using 20 ml of the mixture of methanol and ethanol at 50 min of extraction time. This study also conducted that mixture of methanol and ethanol was a better solvent on improving the ultrasound assisted extraction, as indicated by high protein recovery with less amount of solvent volume used.
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