Abstract
Author SummaryMany protein families are so diverse that it is hard to determine their ancestral functions and to understand how their derived functions evolved. The existence of so many different functions within protein families often creates the impression that complex, novel functions must have evolved repeatedly and independently. Nuclear receptors (NRs) are a large family of related proteins that regulate key biological processes in animals by binding to specific DNA sequences and triggering expression of nearby target genes. Many NRs are activated by a specific hormone or other small molecule, but some do not require a ligand, and still others are incapable of activating gene expression and so act primarily as repressors of transcription. To understand how the functional diversity of NRs evolved, we reconstructed the structural and functional characteristics of the ancient protein from which the entire family evolved, using genomic, biochemical, functional, and structural analyses in a phylogenetic framework. We show, contrary to current belief, that the ancestral NR was a ligand-activated transcriptional activator that existed in the earliest period of animal evolution. Our analysis reveals how the extraordinary functional diversity of modern receptors was generated by subtle tinkering with this ancestral template—slightly reshaping the ligand cavity, stabilizing the protein's active conformation so it no longer required a ligand, or disabling the protein's capacity to activate transcription without affecting its other properties. We predict that, when sufficient data are gathered to allow detailed evolutionary reconstructions in other protein families, it will become apparent that most protein functional diversity evolved by tinkering with ancient functions; invoking the evolution of wholesale “novelty” will seldom be necessary.
Highlights
By sequencing genomes of taxa occupying key positions in the metazoan tree of life, it has become possible to infer when important animal gene families originated and proliferated [1,2,3]
Such a strategy would be analogous to detailed studies of the evolution of animal development, which have revealed the deep homology of diverse morphologies in distant lineages and the mechanisms by which they evolved from common ancestral forms [4]
Many nuclear receptors (NRs) are activated by a specific hormone or other small molecule, but some do not require a ligand, and still others are incapable of activating gene expression and so act primarily as repressors of transcription
Summary
By sequencing genomes of taxa occupying key positions in the metazoan tree of life, it has become possible to infer when important animal gene families originated and proliferated [1,2,3]. It should be possible to reconstruct the history of a protein family by phylogenetically analyzing the underlying structural mechanisms that produce functional diversity among densely sampled members of the family Such a strategy would be analogous to detailed studies of the evolution of animal development, which have revealed the deep homology of diverse morphologies in distant lineages and the mechanisms by which they evolved from common ancestral forms [4]. The second class of NRs are ligand-independent transcription factors, often called ‘‘constitutive’’ receptors, the LBDs of which can adopt the active conformation and activate gene expression in the absence of a ligand or other modifications. Some members of this class lack the internal cavity and are not known to bind any ligands, whereas others do bind hydrophobic
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
