Abstract
A 2-hour lecture summarising key principles, methods and applications of protein engineering techniques. Illustrated with examples from medicine, agriculture, research, bioenergy, and industry. The material is split into two main sections.1) Rational design - including manual, bioinformatic and computational techniques for protein modification and de novo design.2) Directed evolution - covering different systems for generating genetic diversity, selection and screening systems, and linking phenotype to genotype.Finally some hybrid techniques that combine the two approaches are briefly explained.Feel free to reuse any of the slides. All material is referenced at the bottom each slide and notes are provided for most slides with additional info.
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