Abstract
A protein fluorescent sensor array based on variously charged dendritic fluorophores is developed. The variation of charge is achieved by different combinations of cationic trimethylammonium, anionic carboxylate and non-ionic methyl ester or N,N-dimethylamino on the peripheries of the fluorophores. Nine phenylene–ethynylene dendritic fluorophores, seven zeroth generation bearing charges varied from −3 to +3 and two first generation bearing −6 and +6 charges, are synthesized from triiodotriphenylamine through series of Sonogashira coupling. In phosphate buffer saline pH 7.4, five out of these nine compounds fluorogenically response to eight protein analytes distinctively. The data set of fluorescent intensities obtained from 5 fluorophores × 8 proteins × 9 replicated measurements can be statistically sorted into eight clusters corresponding to each protein by principal component analysis (PCA). Factorial discriminant analysis (FDA) cross-validation is applied to locate the optimum detection wavelength and reduce the number of sensing elements from nine to two with 100% discriminating accuracy. The method described should be generally practical for the development of electronic tongue for protein related food analysis and medical diagnosis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.