Abstract
Laminar flow in an open tube leads to solute band spreading. This process is opposed by radial diffusion. The translational diffusion coefficient of a solute can be calculated from the band dispersion, tube length, and flow rate. No calibration is necessary. A simple apparatus was used to measure the diffusion coefficients of several small solutes and 18 proteins. Most results were within ±10% of literature values. Each determination required 7–90 min, depending on the magnitude of the diffusion coefficient. The amount of protein needed was approximately 25 μg. The diffusion coefficient data were useful for the estimation of molecular weights.
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