Abstract
The effect of macromolecular crowding on protein diffusion has been extensively studied in order to understand the protein dynamics in cellular environments. Previous studies have generally focused on proteins as crowding agents as they are known to occupy 20-30% of the cell volume. The volume fraction of the nucleoid in bacterial cells is also significant, around 10-20%, but the effect of chromosomal DNA as a crowding agent on protein diffusion has drawn relatively limited attention. Using recently build high-resolution models of bacterial nucleoids, Brownian and Stokesian dynamics simulations of coarse-grained model systems containing chromosomal DNA and proteins were carried out to investigate the effect of nucleoid crowding on protein diffusion. The coarse-grained model for the nucleoid is an experimentally-derived 3D model of the Caulobacter crescentus chromosome at base-pair resolution built by combining topological information of chromosomal DNA with distance restraints obtained from high-throughput Chromosome Conformation Capture (HiC) experiments, while proteins in the systems are modeled as hard-spheres. Results show that diffusion of proteins are slower around the nucleoid compared to in protein crowding and that there is size-dependent exclusion of proteins from the chromosome interior. Further analysis was carried to examine the degree of anomalous diffusion at different time and length scales.
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