Abstract
The use in protein crystal growth of the newly described non-detergent solubilizers/stabilizers sulfobetaines (NDSB) has been investigated with crystallographic methods. In the classic model of egg white lysozyme crystallization by NaCl, NDSB increased the protein solubility and the crystal growth rate without influencing the quality of crystals as shown by Wilson plots. Sulphur anomalous X-ray scattering was used to show the absence of any bound NDSB in the crystals. A practical example of the use of NDSB is given for Desulfovibrio Gigas type II ferrodoxin, where NDSB contributed to reduction in crystal twinning and resulted in the growth of a new crystal form. With their unique combination of solubilizing and stabilizing properties, the NDSB molecules might prove to be valuable additives for protein crystal growth.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
