Protein coding regions prediction by fusing DNA shape features

Abstract

Exons crucial for coding are often hidden within introns, and the two tend to vary greatly in length, which results in deep learning-based protein coding region prediction methods often performing poorly when applied to more structurally complex biological genomes. DNA shape information also plays a role in revealing the underlying logic of gene expression, yet current methods ignore the influence of DNA shape features when distinguishing coding and non-coding regions. We propose a method to predict protein-coding regions using the CNNS-BRNN model, which incorporates DNA shape features and improves the model's ability to distinguish between intronic and exonic features. We use a fusion coding technique that combines DNA shape features and traditional sequence features. Experiments show that this method outperforms the baseline method in metrics such as AUC and F1 by 2.3% and 5.3%, respectively, and the fusion coding method that introduces DNA shape features has a significant improvement in model performance.