Protein chiral selectors in free-solution capillary electrophoresis and packed-capillary electrochromatography

Abstract

The use of proteins as chiral selectors in CE is reviewed. The performance of packed-capillary electrochromatography with protein phases is compared with free-solution systems. The use of free-solution CE with protein additives for the determination of protein-ligand binding and ligand-ligand interactions is discussed. Some new results are presented using capillaries packed with immobilized human serum albumin. The measurement by free-solution CE of binding of three cationic phenothiazine derivatives to human serum albumin is also shown, and the potential and pitfalls of this method are discussed. Finally some data on the use of dextran to modify protein mobility are shown, and effects of the dextran co-additive on the binding of some ligands to human serum albumin are shown.