Protein changes induced by cadmium stress in three radish cultivar seedlings: a comparison using two-dimensional gel electrophoresis

Abstract

The germination rate was decreased to 92% when seeds were treated at 0.5 mmol·L -1 Cd 2+ and was progressively decreased with higher Cd 2+. 83% and 67% germination rates were observed respectively at 1 mmol·L -1 and 5 mmol·L -1 Cd 2+ treatment in XWY, but the lower germination rate was observed with 5 mmol·L -1 Cd 2+ treatment in XYB. The germination rate was decreased to 83% only at 0.05 mmol·L -1 Cd 2+ and only 58% germination rate was observed when seeds were treated at 1 mmol·L -1 and 5 mmol·L -1 Cd 2+ in WQ. The effects of Cd 2+ on seedling growth were observed in three radish cultivars. Seedling growth damage was much more serious with higher Cd 2+ concentration. WQ is the most sensitive to Cd 2+ treatment according to the experiment results which were obtained by testing germination rate and seedling growth in three radish cultivars. 5 protein spots were disappeared and 15 new protein spots were induced in XWY seedling which were treated by 0.1 mmol·L -1 Cd 2+, 2 protein spots were disappeared and 1 protein spot was decreased in content, 13 new protein spots were induced in XYB, 12 new protein spots were induced in WQ. A close positive relationship was observed between protein spots change and inhibited seedling growth in the three radish cultivars which were treated by 0.1 mmol·L -1Cd 2+. It may be important for understanding biochemical mechanism of Cd 2+ damage.