Abstract
Abstract We describe a relatively simple, specific, and practicable method for measuring plasma testosterone by competitive protein-binding analysis. An ether extract is purified by a single chromatographic step on a micro-scale Celite—ethylene glycol column. Serum from women in the third trimester of pregnancy is used as the binding protein, and an ammonium sulfate precipitation step is used to separate the free and protein-bound testosterone. The method has a consistently low blank [0.59 ± 0.26 (SD) pmol/sample] and shows good precision. The mean testosterone concentration in normal men and menstruating women was 17.1 ± 4.0 (SD) and 1.2 ± 0.4 (SD) nmol/liter, respectively.
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