Abstract
In plant cells the plasma membrane is a highly elaborated structure that functions as the point of exchange with adjoining cells, cell walls and the external environment. In this study, we investigated the structure and function characteristic of wheat root plasma membrane (PM) as affected by H2O2 and Fe by using fluorescence spectroscopic and attenuated total reflectance infrared (ATR-IR) techniques. The results showed that these oxidant damaged induced an obviously reduced membrane fluidity were observed in the roots PM treated with the 200 μM H2O2, FeSO4, and FeCl3. Computer-aided software analyses of the FTIR spectrum indicated that the content of the α-helices decreased and β-sheet increased in the secondary structures of proteins after exposure to the oxidants of 200 μM H2O2, FeSO4, and FeCl3. The number of P=O and C=C bonds area declined rapidly in the lipids of the membrane under the oxidants stress. These structural alterations might explain the reason of the roots PM instability under most of the abiotic stress.
Highlights
Plant cell membrane is the thin layer of protein and fat that surrounds the cell
It was showed that the ANS fluorescence intensity of treated with 200 μM H2O2 was almost double times than that untreated the roots plasma membrane (PM) vesicles, but the fluorescence intensity values of the roots PM treated with 200 μM Fe2+ and Fe3+ were appeared a little bit higher than that of untreated the roots PM vesicles, indicating a little bit stronger of damage
Using band area measure tool of OMIC softerware, we found from Figure 2(b) that the Amide I content of the roots PM in Fe2+, H2O2, and Fe3+ treated only remain of 87%, 33%, a Fluorescence intensity(unit)
Summary
Plant cell membrane is the thin layer of protein and fat that surrounds the cell. The cell membrane is semi permeable, allowing some substances to pass into the cell and blocking others. Most transport proteins in plant cells are energized by electrochemical gradients of protons across the plasma membrane. ROS includes singlet oxygen ( O12 ), peroxide (H2O2) and shuypderrooxxyidl erardadiciaclal(H( OO2 −))., hydrogen They are considered to be toxic byproducts of aerobic metabolism because they can react with various cellular components to induce oxidative damage [1]. Current evidence suggests that OH and H2O2 cause damage to cells by the generation of potent oxidizing species. Previous studies [2,3,4] have indicated that oxygen radicals and other activated oxygen species such as H2O2 can increase protein degradation in intact cells and intracellular organelles, as well as in vitro systems. OH and H2O2 are known to damage various proteins by inducing oxidative modification, non-enzymatic fragmentation, and aggregation, or indirectly by peroxidation of membrane phospholipids [2]
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