Abstract
Typical porous silica (SBA-15) has been modified with pore expander agent (1,3,5-trimethylbenzene) and fluoride-species to diminish the length of the channels to obtain materials with different textural properties, varying the Si/Zr molar ratio between 20 and 5. These porous materials were characterized by X-ray Diffraction (XRD), N2 adsorption/desorption isotherms at −196 °C and X-ray Photoelectron Spectroscopy (XPS), obtaining adsorbent with a surface area between 420–337 m2 g−1 and an average pore diameter with a maximum between 20–25 nm. These materials were studied in the adsorption of human blood serum proteins (human serum albumin—HSA and immunoglobulin G—IgG). Generally, the incorporation of small proportions was favorable for proteins adsorption. The adsorption data revealed that the maximum adsorption capacity was reached close to the pI. The batch purification experiments in binary human serum solutions showed that Si sample has considerable adsorption for IgG while HSA adsorption is relatively low, so it is possible its separation.
Highlights
In spite of the porous silica having been evaluated in a plethora of biomolecules, these adsorbents have not been studied to adsorption of typical human serum proteins, such as human serum albumin and immunoglobulins, yet
Protein bands were developed by Coomassie Blue. It has been synthesized a set of porous silica, which have been modified to increase the pore diameter and reduce the length of the pore in order to favor the hosting of bulky biomolecules such as Immunoglobulin G (IgG) and Human serum albumin (HSA)
The analysis of the adsorption capacity at different buffers has indicated that the maximum adsorption capacity takes place at a pH close to the pI, since under these pH
Summary
The selective immobilization and releasing of biomolecules, such as proteins, enzymes or oligonucleotides in porous structures and/or on the surface of nanoparticles, has emerged as an interesting process in several fields, such as biocatalysts [1,2,3], bioengineering [1,2,3], biosensing [4,5], separation by chromatographic techniques [6,7,8] or drug delivery [9,10,11]. The adsorption capacity of the porous silica can be modified by the incorporation of heteroatoms, such as Al [66,67] or Zr [46,68], since the substitution of Si atoms by Al or Zr atoms modifies the electronic density of the porous silica and generates acid sites This fact can improve the interaction between the porous structure and the biomolecules as previously indicated several authors [66,68]. In spite of the porous silica having been evaluated in a plethora of biomolecules, these adsorbents have not been studied to adsorption of typical human serum proteins, such as human serum albumin and immunoglobulins, yet. Binary mixture with IgG and HSA were mixed in different proportions to evaluate the adsorption capacity by electrophoreses
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
