Protein adsorption on spark plasma sintered 2d-, 3d- and lamellar type mesoporous silicate compacts.

Abstract

The preparation of lamellar type mesoporous silica (MPS) compact through the spark plasma sintering (SPS) and the adsorption/desorption of protein onto MPS compact are reported to be compared with those onto 2d‐hexagonal and 3d‐cubic type MPS compacts. A lamellar‐type MPS powder (MPS‐la) was prepared using triblock copolymer, PEO5 PPO68 PEO5, and was compacted in a carbon die and heated at 500°C for 5 min under uniaxial pressure. The products are referred to as MPS‐la‐500. The MPS compacts keep the lamellar type mesoporous configuration. The adsorbed amount of protein onto MPS‐la‐500 was 100 mg/g, while that on MPS‐la was 130 mg/g, and the former decreased by 23%. However, its decreasing ratio of the protein adsorption on MPS‐la‐500 was less than those of 2d‐hexagonal and 3d‐cubic type MPS compacts, which were 73 and 34%, respectively. The released amount of protein into PBS solution from MPS‐la‐500, which was soaked in the protein solution for 48 h, increased with the soaking time, while those from 2d‐ and 3d‐type MPS compacts reached to plateau for 4 h of soaking. The lamellar type MPS compact was found to be easier to absorb and release proteins, which may be due to the large aperture of the mesoporous configuration.