Abstract
The author's work on the adsorption of plasma proteins using radiolabeling, hydrodynamic thickness measurements and elution/identification methods is reviewed. This work was motiviated by the need to understand the role of protein adsorption in thrombus formation following blood-foreign surface contact. Studies of single protein systems showed that adsorption occurs in monolayers, that adsorption is inherently, but only slowly reversible and that some alterations of protein structure can occur upon adsorption. Work with protein mixtures showed the strong preferential adsorption of fibrinogen relative to albumin and IgG. From plasma there is a relative lack of adsorption of the abundant proteins. Adsorption of fibrinogen from plasma was shown to be transient, it is displaced by contact phase clotting proteins. Part of the fibrinogen adsorbed from plasma was found to be degraded by surface generated plasmin implying that surfaces have thrombolytic as well as thrombogenic properties.
Published Version
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