Abstract
In this report, fluorescence detection coupled capillary electrophoresis (CE-FL) was used to detect Protein A. Antibody was first labeled with Cy5 and then mixed with quantum dots (QDs) to form QDs-antibody bioprobe. Further, we observed fluorescence resonance energy transfer (FRET) from QDs donor to Cy5 acceptor. The bioprobe was formed and brought QDs and Cy5 close enough to allow FRET to occur. After adding protein A, the FRET system was broken and caused the FRET signal to decrease. Thus, a new method for the determination of protein A was proposed based on the FRET signal changes. This study provides a new trail of thought for the detection of protein.
Highlights
Compared with traditional organic dyes, the quantum dots (QDs) developed in recent years have many attractive features, including high photobleaching threshold, good chemical stability, relatively narrow and symmetric luminescence bands [1]
Combine capillary electrophoresis (CE) and fluorescence resonance energy transfer (FRET) technology, we have studied the interaction of protein and nanoparticles [15], the protein peptide interaction [16] and enzyme detection [17], etc
QDs and IgG-Cy5 mixtures were first analysed by CE-FL
Summary
Compared with traditional organic dyes, the QDs developed in recent years have many attractive features, including high photobleaching threshold, good chemical stability, relatively narrow and symmetric luminescence bands [1]. Many strategies have been developed to conjugate QDs with biomolecules for the preparation of QDs based bioprobes, for example: covalent conjugation method [5], metal-affinity driven self-assembly method [6,7] and electrostatic adsorption method [8,9]. The bioprobes prepared in this method can be used to detect biomolecules. We prepared QDs-IgG-Cy5 bioprobe by electrostatic adsorption method. With adding protein A, the FRET signals decrease gradually and this method can be used for protein A detection
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