Abstract
Hos3 is involved in cellular growth under osmotic stress in Schizosaccharomyces pombe. The recombinant plasmid pYFHos3 harboring the structural gene encoding Hos3 was constructed. The S. pombe cells harboring pYFHos3 contained the increased hos3 (+) mRNA content and exhibited an enhanced growth in high osmotic conditions, such as 1.5M KCl and 2.5M D-glucose, compared with the vector control cells. In the presence of hydrogen peroxide (H2O2), superoxide anion-generating menadione (MD) and nitric oxide (NO)-generating sodium nitroprusside (SNP), they could grow better than the vector control cells. In the presence of H2O2, MD and SNP and in the absence of a nitrogen source, the S. pombe cells harboring pYFHos3 contained less elevated NO and reactive oxygen species (ROS) levels than the vector control cells. Collectively, the S. pombe Hos3 also participate in the cellular defense against oxidative, nitrosative and nutritional stresses through down-regulating ROS and NO levels.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
