Protective role of ginseng extract against oxidative stress, reproductive and some biochemical parameters alterations induced by doxorubicin in male rats

Mohammed Kassem,Abdel-Fattah Ali,Seham Y Abo-Kora,Nesreen Shawky

doi:10.14419/ijpt.v8i1.30667

Abstract

This study investigates the modulating effect of ginseng against testicular toxicity, oxidative stress and changes in some biochemical parameters induced by doxorubicin. Twenty male rats were divided into four groups. The 1st group received distilled water orally (control group), The 2nd group received doxorubicin (5 mg/kg b.wt. intrapertenoineal) once a week for eight weeks, The 3rd group received ginseng extract (200 mg/kg b.wt.) daily for eight weeks and the 4th group received doxorubicin with ginseng extract by the same doses as in the 2nd and the 3rd groups respectively. At the end of the 8th week, blood and semen samples were taken for biochemical and semen analysis, respectively. The doxorubicin treated group had significantly higher serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), Creatine kinase (CK) and lactate dehydrogenase (LDH) along with lower levels of total protein, albumin and globulin. In addition, a significant decrease in antioxidant enzymes (SOD, CAT, GSHPx), and glutathione (GSH) associated with higher level of malondialdehyde (MDA) were observed. At the same time, the group that took doxorubicin with ginseng did not differ from control group in terms of these parameters. Male fertility study showed changes in testosterone and semen analysis in both groups treated with doxorubicin, while the group that took doxorubicin with ginseng showed an improvement towards control levels of these parameters. Thus ginseng supplement can reduce the negative effects of doxorubicin- induce.Â Â

Highlights

Doxorubicin is antibiotic drug isolated from Streptomyces peucetius var. caesius (Arcamone et al 1969, Frederick et al 1990) which is first generation of anthracycline, a potent broad spectrum chemotherapeutic agent used for the treatment of several types of cancer (Sakr et al 2013, Chen et al 2016)
Regarding the changes in biochemical parameters, there was a significant increase in AST, ALT, lactate dehydrogenase (LDH) and Creatine kinase (CK) activities along with a significant decrease in total protein, albumin and globulin levels in doxorubicin group in comparison with control group
There was a significant decrease in AST, ALT, LDH and CK activities associated with non-significant changes in total protein and albumin levels in doxorubicin plus ginseng group when compared with doxorubicin group

Summary

Introduction

Doxorubicin is antibiotic drug isolated from Streptomyces peucetius var. caesius (Arcamone et al 1969, Frederick et al 1990) which is first generation of anthracycline, a potent broad spectrum chemotherapeutic agent used for the treatment of several types of cancer (Sakr et al 2013, Chen et al 2016). Caesius (Arcamone et al 1969, Frederick et al 1990) which is first generation of anthracycline, a potent broad spectrum chemotherapeutic agent used for the treatment of several types of cancer (Sakr et al 2013, Chen et al 2016). It is used in treatment of breast, uterine, ovarian, bladder and lung cancers (Marinelo et al 2018). It was found that some herbal nutrients reduce the toxic effect of doxorubicin through its antioxidant effect (Shihong et al 2014). The objective of the present work is to evaluate the effect of doxorubicin on male fertility and biochemical parameters in rats and modulating effect of ginseng extract

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Conclusion