Abstract

Aim:The major objective of the investigation was to evaluate the hitherto uncharacterized potential of Brucella-specific antibodies to win the battle against virulent Brucella abortus infection.Materials and Methods:Brucella-specific immune serum was raised in mice. The antibody titer of serum was determined by standard tube agglutination test and indirect enzyme-linked immunosorbent assays (iELISA). Groups of mice and guinea pigs were passively immunized with serum containing specific agglutinin titers. 24 h after immunization, all animals along with unimmunized controls were challenged with B. abortus S544. Total B. abortus S544 counts in the spleen of each animal collected on the 7th day of challenge was determined to evaluate the protective index (PI) of anti-Brucella serum by statistical analysis.Result:A dose-dependent protective response to immune mice serum was observed in both experimental models though the values of PI of mice were higher than those obtained for guinea pigs. The PI values in mice passively immunized with 50 IU or 25 IU antibodies were 1.38 and 0.69, respectively. In guinea pigs, however, animals passively immunized with 50 IU or 25 IU antibodies showed PI values equivalent to 0.79 and 0.41, respectively.Conclusion:The observations support our hypothesis that the presence of antibodies inhibits the initial multiplication and eventual colonization of systemic organs by B. abortus. Therefore, a predominant antibody-mediated response induced by a vaccine is expected to protect the animal against the most severe clinical outcome of infection.

Highlights

  • Brucella abortus is one of the most common zoonotic microbes that affect both bovine and human population

  • A dose-dependent protective response to immune mice serum was observed in both experimental models though the values of protective index (PI) of mice were higher than those obtained for guinea pigs

  • The PI values in mice passively immunized with 50 IU or 25 IU antibodies were 1.38 and 0.69, respectively

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Summary

Introduction

Brucella abortus is one of the most common zoonotic microbes that affect both bovine and human population. It is Gram-negative, alphaproteobacteria that impart an enormous burden on the Indian economy due to abortion, infertility, reduced calf, and milk production in livestock and pose a serious impact on public health [1]. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated

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